Study Report
Reference
| Citation | Chen, 2010 PubMed |
| Full Info | Chen, Y., Jiang, T., Chen, P., Ouyang, J., Xu, G., Zeng, Z. and Sun, Y. (2010) Emerging tendency towards autoimmune process in major depressive patients: A novel insight from Th17 cells. Psychiatry Res.
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Study
| Hypothesis or Background |
Evidence indicates that there is an emerging tendency towards autoimmunity occuring in major depressive disorder (MDD). The aim of our study is to investigate the mechanism of autoimmune process in MDD from a novel insight of Th17 cells, which have been identified as the significant activators of autoimmunity.
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| Sample Information | 40 patients with MDD and 30 healthy control subjects |
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| Method Detail | Indirect immunofluorescence test; flow cytometric analysis; RT-PCR; ELISA |
| Method Keywords | immunocytochemical analysis; flow cytometric analysis; RT-PCR; Enzyme-linked immunosorbent assay (ELISA) |
| Result | Indirect immunofluorescence test was used for detection of serum antinuclear antibody (ANA), showing that patient group was positive more frequently than control group. By flow cytometric analysis, depressed subjects revealed a significant increase in peripheral Th17 cell number, and an obvious decrease in T-reg cell number, showing an imbalance of Th17/Treg ratio compared to healthy controls. We also found a higher level of RORgammat (retinoic acid-related orphan receptor-gammat, the specific transcription factor of Th17 cell) mRNA expression in peripheral blood lymphocytes by RT-PCR and serum concentration of IL-17 by ELISA in patients. |
| Conclusions | In conclusion, our study displayed a potential role of Th17 cells in autoimmune process in MDD patients, contributing to the existing evidence of autoimmune inclination in MDD. |
Relationships reported by
Chen, 2010
| Component A Approved Name (Name in Paper) |
Component A Type |
Component B Approved Name (Name in Paper) |
Component B Type |
Statistical Result |
Relationship Description |
Result Category (Positive/Negative)) |
|
MDD
|
syndrome |
T-Lymphocytes, Regulatory (T-reg cell) |
cell |
|
depressed subjects revealed an obvious decrease in T-reg cell numbe |
Positive
|
|
MDD
|
syndrome |
Th17 Cells (Th17) |
cell |
|
depressed subjects revealed a significant increase in peripheral Th17 cell number |
Positive
|
|
MDD
|
syndrome |
RORC (RORgammat (retinoic acid-related orphan receptor-gammat)) |
gene |
|
A higher level of RORgammat (retinoic acid-related orphan receptor-gammat, the specific transcription factor of Th17 cell) mRNA expression in peripheral blood lymphocytes was found in patient. |
Positive
|
|
MDD
|
syndrome |
Interleukin-17A (IL17) |
protein |
|
A higher level of serum concentration of IL-17 was found in patient. |
Positive
|
