Study Report
Reference
| Citation | Ren, 2011 PubMed |
| Full Info | Ren, X., Dwivedi, Y., Mondal, A.C. and Pandey, G.N. (2011) Cyclic-AMP response element binding protein (CREB) in the neutrophils of depressed patients. Psychiatry Res, 185, 108-112.
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Study
| Hypothesis or Background |
Cyclic-AMP response element binding (CREB) protein regulates the expression of many genes involved in the pathophysiology of depression. Increased CREB levels were found in the brain of antidepressant-treated rats and decreased protein and mRNA expression of CREB was reported in the postmortem brain of depressed suicide victims.
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| Sample Information | neutrophils obtained from 22 drug-free patients with major depressive disorder (MDD) and 23 normal control subjects |
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| Method Detail | We determined CREB protein expression, using Western blot technique, and CRE-DNA binding, using gel shift assay, in neutrophils obtained from 22 drug-free patients with major depressive disorder (MDD) and 23 normal control subjects. Diagnosis of patients was based on Diagnostic and Statistical Manual of Mental Disorders DSM-IV criteria; severity of illness was rated by Hamilton Depression Rating Scale (HDRS). |
| Method Keywords | postmortem study; immunoblotting; gel shift assay |
| Result | We found that the CRE-DNA binding activity and CREB protein expression were significantly decreased in the neutrophils of drug-free MDD patients compared with normal control subjects. |
| Conclusions | Our findings suggest that CREB may play an important role in the pathophysiology of depression and that it may be an important target for the therapeutic action of antidepressant drugs. Neutrophil CREB levels may also serve as a useful biomarker for patients with MDD. |
Relationships reported by
Ren, 2011
| Component A Approved Name (Name in Paper) |
Component A Type |
Component B Approved Name (Name in Paper) |
Component B Type |
Statistical Result |
Relationship Description |
Result Category (Positive/Negative)) |
|
MDD
|
syndrome |
Cyclic AMP-responsive element-binding protein 1 (CREB) |
protein |
|
We found that the CRE-DNA binding activity and CREB protein expression were significantly decreased in the neutrophils of drug-free MDD patients compared with normal control subjects. |
Positive
|
|
MDD
|
syndrome |
Cyclic AMP-responsive element-binding protein 1 (Cyclic-AMP response element binding (CREB) protein) |
protein |
|
The CRE-DNA binding activity and CREB protein expression were significantly decreased in the neutrophils of drug-free MDD patients compared with normal control subjects. |
Positive
|
|
MDD
|
syndrome |
Leukocytes (neutrophils) |
cell |
|
We found that the CRE-DNA binding activity and CREB protein expression were significantly decreased in the neutrophils of drug-free MDD patients compared with normal control subjects. |
Positive
|
