Study Report
Reference
| Citation | Johnson, 2011 PubMed |
| Full Info | Johnson, S., Stockmeier, C.A., Meyer, J.H., Austin, M.C., Albert, P.R., Wang, J., May, W.L., Rajkowska, G., Overholser, J.C., Jurjus, G. et al. (2011) The Reduction of R1, a Novel Repressor Protein for Monoamine Oxidase A, in Major Depressive Disorder. Neuropsychopharmacology.
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Study
| Hypothesis or Background |
The novel transcriptional repressor protein, R1 (JPO2/CDCA7L/RAM2), inhibits monoamine oxidase A (MAO A) gene expression and influences cell proliferation and survival. MAO A is implicated in several neuropsychiatric illnesses and highly elevated in major depressive disorder (MDD); however, whether R1 is involved in these disorders is unknown.
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| Sample Information | 18 untreated MDD subjects and 12 medicated MDD subjects compared with 18 matched psychiatrically normal control subjects |
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| Method Detail | This study evaluates the role of R1 in depressed subjects either untreated or treated with antidepressant drugs. R1 protein levels were determined in the postmortem prefrontal cortex of 18 untreated MDD subjects and 12 medicated MDD subjects compared with 18 matched psychiatrically normal control subjects. |
| Method Keywords | postmortem study; immunoblotting |
| Result | Western blot analysis showed that R1 was significantly decreased by 37.5% (p<0.005) in untreated MDD subjects. The R1 level in medicated MDD subjects was also significantly lower (by 30%; p<0.05) compared with control subjects, but was not significantly different compared with untreated MDD subjects. Interestingly, the reduction in R1 was significantly correlated with an increase (approximately 40%; p<0.05) in MAO A protein levels within the MDD groups compared with controls. Consistent with the change in MAO A protein expression, the MAO A catalytic activity was significantly greater in both MDD groups compared with controls. These results suggest that reduced R1 may lead to elevated MAO A levels in untreated and treated MDD subjects; moreover, the reduction of R1 has been implicated in apoptotic cell death and apoptosis has also been observed in the brains of MDD subjects. |
| Conclusions | Therefore, modulation of R1 levels may provide a new therapeutic target in the development of more effective strategies to treat MDD. |
Relationships reported by
Johnson, 2011
| Component A Approved Name (Name in Paper) |
Component A Type |
Component B Approved Name (Name in Paper) |
Component B Type |
Statistical Result |
Relationship Description |
Result Category (Positive/Negative)) |
|
Amine oxidase [flavin-containing] A (monoamine oxidase A)
|
protein |
Ribonucleoside-diphosphate reductase large subunit (R1) |
protein |
P-value<0.05 |
Western blot analysis showed that R1 was significantly decreased by 37.5% (p<0.005) in untreated MDD subjects. The R1 level in medicated MDD subjects was also significantly lower (by 30%; p<0.05) compared with control subjects, but was not significantly different compared with untreated MDD subjects. Interestingly, the reduction in R1 was significantly correlated with an increase (approximately 40%; p<0.05) in MAO A protein levels within the MDD groups compared with controls. |
Positive
|
|
MDD
|
syndrome |
Ribonucleoside-diphosphate reductase large subunit (R1) |
protein |
P-value<0.005 |
Western blot analysis showed that R1 was significantly decreased by 37.5% (p<0.005) in untreated MDD subjects. The R1 level in medicated MDD subjects was also significantly lower (by 30%; p<0.05) compared with control subjects, but was not significantly different compared with untreated MDD subjects. Interestingly, the reduction in R1 was significantly correlated with an increase (approximately 40%; p<0.05) in MAO A protein levels within the MDD groups compared with controls. |
Positive
|
|
MDD
|
syndrome |
Amine oxidase [flavin-containing] A (MAO A) |
protein |
P-value<0.05 |
An increase (approximately 40%; p<0.05) in MAO A protein levels within the MDD groups compared with controls, the MAO A catalytic activity was significantly greater in both MDD groups compared with controls |
Positive
|
